Title: A role for NuSAP in linking microtubules to mitotic chromosomes
Authors: Ribbeck, Katharina ×
Raemaekers, Tim
Carmeliet, Geert
Mattaj, Iain W #
Issue Date: 6-Feb-2007
Publisher: Current Biology Ltd.
Series Title: Current Biology vol:17 issue:3 pages:230-236
Abstract: The spindle apparatus is a microtubule (MT)-based machinery that attaches to and segregates the chromosomes during mitosis and meiosis. Self-organization of the spindle around chromatin involves the assembly of MTs, their attachment to the chromosomes, and their organization into a bipolar array. One regulator of spindle self-organization is RanGTP. RanGTP is generated at chromatin and activates a set of soluble, Ran-regulated spindle factors such as TPX2, NuMA, and NuSAP [1]. How the spindle factors direct and attach MTs to the chromosomes are key open questions. Nucleolar and Spindle-Associated Protein (NuSAP) was recently identified as an essential MT-stabilizing and bundling protein that is enriched at the central part of the spindle [2, 3]. Here, we show by biochemical reconstitution that NuSAP efficiently adsorbs to isolated chromatin and DNA and that it can directly produce and retain high concentrations of MTs in the immediate vicinity of chromatin or DNA. Moreover, our data reveal that NuSAP-chromatin interaction is subject to Ran regulation and can be suppressed by Importin alpha (Imp alpha) and Imp7. We propose that the presence of MT binding agents such as NuSAP, which can be directly immobilized on chromatin, are critical for targeting MT production to vertebrate chromosomes during spindle self-organization.
ISSN: 0960-9822
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical and Experimental Endocrinology
Molecular Genetics Section (-)
Biomedical Sciences Group Management - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
K Ribbeck et Al, 2007, Current Biology,17; 230-236.pdf Published 843KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science