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Title: Streptomyces as host for recombinant production of Mycobacterium tuberculosis proteins
Authors: Vallin, C ×
Ramos, A
Pimienta, E
Rodriguez, C
Hernandez, T
Hernandez, L
Del Sol, R
Rosabal, Grisel
Van Mellaert, Lieve
Anné, Jozef #
Issue Date: May-2006
Publisher: Churchill Livingstone
Series Title: Tuberculosis vol:86 issue:3-4 pages:198-202
Conference: International Workshop on Tuberculosis Vaccines location:Varadero, Cuba date:8-12 May 2005
Abstract: The 45/47kDa APA protein (110860) of Mycobacterium tuberculosis was produced by Streptomyces lividans. The recombinant protein could be recovered from the culture medium of an S. lividans clone containing the apa gene under control of the promoter and signal sequence of the Streptomyces coelicolor agarase gene. The recombinant protein production was further scaled-up using fermentation conditions. The APA protein was subsequently purified from the culture supernatant by means of immunochromatography. About 80mg of recombinant protein were obtained per liter of culture media.
URI: 
ISSN: 1472-9792
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Bacteriology (Rega Institute)
× corresponding author
# (joint) last author

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