International Workshop on Tuberculosis Vaccines location:Varadero, Cuba date:8-12 May 2005
The 45/47kDa APA protein (110860) of Mycobacterium tuberculosis was produced by Streptomyces lividans. The recombinant protein could be recovered from the culture medium of an S. lividans clone containing the apa gene under control of the promoter and signal sequence of the Streptomyces coelicolor agarase gene. The recombinant protein production was further scaled-up using fermentation conditions. The APA protein was subsequently purified from the culture supernatant by means of immunochromatography. About 80mg of recombinant protein were obtained per liter of culture media.