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Title: Candida albicans biofilm formation in a new in vivo subcutaneous model
Authors: Ricicova, Marketa ×
Kucharikova, Sona
Tournu, Hélène
Lagrou, Katrien
Van Dijck, Patrick #
Issue Date: Mar-2008
Conference: ASM Candida and Candidiasis meeting edition:9th location:New Jersey, USA date:March 24-28th 2008
Abstract: Microorganisms are often found in nature as structured communities attached to surfaces, commonly referred to as biofilms. Biofilm formation caused by the opportunistic fungal pathogen Candida albicans has significant clinical consequences, as adhesion to inert surfaces such as urinary or central venous catheters, dental prostheses, and other biomaterials can lead to a failure of these implanted devices.
Candida biofilms are mainly studied in various in vitro systems using different types of substrate materials, media and conditions. However, in vitro assays do not reflect the influence of the host immune system and the infection site. Here, we describe a new in vivo model which enables to study Candida biofilm formation on pieces of polyurethane catheters implanted under the skin of rats. This model is based on a successful bacterial biofilm model (Van Wijngaerden et al., 1999) and it is optimised for Candida albicans in the commonly used rat strain Sprague Dawley. It comprises of a simple unsophisticated technique which is an advantage compared to the previously published in vivo central venous catheter model (Andes et al., 2004). The new in vivo subcutaneous model allows studying biofilm structure, quantity and gene expression during biofilm formation. Moreover, it can serve as a good tool to test new antifungals. We have studied biofilm development of selected mutants in vivo as well as in vitro and compared the results to other available models.

References:
Van Wijngaerden E, Peetermans WE, Vandersmissen J, Van Lierde S, Bobbaers H, Van Eldere J (1999). Foreign body infection: a new rat model for prophylaxis and treatment. J Antimicrob Chemother. 1999 Nov;44(5):669-74.
Andes D, Nett J, Oschel P, Albrecht R, Marchillo K, Pitula A (2004). Development and characterization of an in vivo central venous catheter Candida albicans biofilm model. Infect Immun. Oct;72(10):6023-31.
Publication status: accepted
KU Leuven publication type: IMa
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Molecular Microbiology and Biotechnology Section - miscellaneous (-)
× corresponding author
# (joint) last author

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