Moss 2008 location:Tampere, Finland date:15-16 August 2008
Trehalose is a non-reducing disaccharide consisting of two glucose molecules linked by 1α–1α bond. It is present in very different organisms such as bacteria, fungi, nematodes, insects and plants. Several functions have been described for trehalose in nature, being a compatible solute, storage compound and structural part of cell walls. So far, five trehalose biosynthesis pathways have been described. Of these, the most widely distributed and best-characterized pathway consists of two steps mediated by the trehalose 6-phosphate synthase (TPS) and trehalose 6-phosphate phosphatase (TPP) enzymes, where the first is catalyzing the synthesis of trehalose 6-phosphate (T6P) while TPP dephosphorylates T6P to form trehalose and Pi. The plant TPS and TPP genes form multigene families where members are divided in classes I, II and III. Recent findings show the importance of trehalose and its intermediate T6P in plant sugar metabolism, gene regulation and development, suggesting T6P as a new plant hormone. In the present work, we characterized the whole trehalose biosynthetic gene familys from Physcomitrella patens, and show that members of the three gene classes are present in Physcomitrella and are being differentially regulated by drought and ABA. We willBy useing theseis member of a primitive plants group as a model to investigate the role in sugar signalling, development and complex gene regulation of the different genes of the large gene family (12 members in P. patens). for trehalose studies, we intend to generate new tools to understand the complex gene regulation in crop plants.