FunNet meeting location:Gosau, Austria date:September 12-15th 2007
The majority of microbes are not found as free-living organisms in their natural habitats but rather in structured communities attached to surfaces, commonly referred to as biofilms. Biofilm formation caused by Candida albicans has significant clinical consequences, as adhesion to inert surfaces such as urinary or central venous catheters, dental prostheses, and other biomaterials can lead to a failure of these implanted devices.
Previous studies have shown that a transcription factor Bcr1 plays an important role in biofilm formation and that a surface protein Als3 is a key target of Bcr1 protein. als3 mutant had a severe defect in biofilm formation in vitro and bcr1 mutant was unable to populate catheter surface neither in vitro nor in vivo (Nobile et al., 2006).
In this study, we test als3 and bcr1 mutants in RPMI medium in vitro and in a new in vivo subcutaneous model and we demonstrate that both strains can form biofilms in both models.