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Title: Candida glabrata Krh proteins can functionally complement the yeast deletion mutant
Authors: De Brucker, Katrijn
Van Dijck, Patrick #
Issue Date: May-2007
Conference: FEBS advanced lecture course on human fungal pathogens edition:2 location:La Colle sur Loup, France date:11-16 May 2007
Abstract: Candida species are considered a major cause of opportunistic infections in humans. Although C. albicans is the most common Candida species, C. glabrata already causes 20% of systemic candidiasis and 30% of urinary tract infections. C. glabrata is resistant to fluconazole, a common used antifungal, and has a higher mortality rate compared to C. albicans. The genome of C. glabrata shows a high degree of homology with S. cerevisiae and is haploid. In contrary to C. albicans, C. glabrata has a normal codon usage.

Recently Peeters and colleagues (2006) discovered a mechanism in yeast by which the G alfa protein Gpa2 activates PKA through two kelch-repeat proteins, Krh1 and Krh2, bypassing adenylate cyclase stimulation. Hence, Gpa2 regulates PKA activity via two distinct pathways: through stimulation of adenylate cyclase and through inhibition of the Krh proteins. We investigated if the C. glabrata homologues of ScKrh1 and ScKrh2 can complement the respective deletion mutants of S. cerevisiae. By measuring the trehalose content of the respective deletions mutants, transformed with CgKrh1 and CgKrh2 cloned into pBEVY-vectors, after 12, 24 and 48 hours, we showed that ChKrh1 and ChKrh2 can complement the function of ScKrh1 and ScKrh2. We will also investigate the expression of STRE-controlled genes and the formation of pseudohyphae in these transformed deletion mutants.

C. glabrata KRH1 and KRH2 deletion strains will be made. The morphology, trehalose mobilisation and the expression of STRE-controlled genes in these mutants will be investigated.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous
# (joint) last author

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