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Title: Measurement of individual angiotensin peptides by HPLC-RIA
Authors: Hanssens, Myriam ×
Keirse, M J
Lijnen, Paul
Kucharski, M J
Luyten, C
Van Assche, Frans Andre #
Issue Date: Jul-1990
Series Title: Journal of biochemical and biophysical methods vol:21 issue:2 pages:103-13
Abstract: Immunoreactive measurements of Angiotensin II in plasma, relate to a variety of angiotensin peptides with different biological activities. A method is described to differentiate these individual angiotensin peptides. It involves extraction of the peptides from plasma by reversible adsorption to phenylsilyl silica cartridges, separation by an isocratic, ion pairing high-pressure liquid chromatography technique and measurement of the appropriate fractions by radioimmunoassay. In umbilical venous plasma molar concentrations of the smaller angiotensin fragments were found to range between 16 and 25% of the concentrations of the angiotensin II octapeptide. Because some angiotensin antisera show higher affinity for the smaller peptides than for the octapeptide, concentrations of angiotensin II, measured by radioimmunoassay, may be overestimated by up to 35% unless the various angiotensin peptides are adequately separated.
ISSN: 0165-022X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pathophysiology of Pregnancy Section (-)
Section Woman - Miscellaneous (-)
Hypertension and Cardiovascular Epidemiology
Faculty of Medicine - miscellaneous
× corresponding author
# (joint) last author

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