Title: In vivo bioluminescence imaging in an experimental mouse model for dendritic cell based immunotherapy against malignant glioma
Authors: Maes, Wim ×
Deroose, Christophe
Reumers, Veerle
Krylychkina, Olga
Gijsbers, Rik
Baekelandt, Veerle
Ceuppens, Jan
Debyser, Zeger
Van Gool, Stefaan #
Issue Date: Jan-2009
Publisher: Springer Netherlands
Series Title: Journal of Neuro-Oncology vol:91 issue:2 pages:127-139
Abstract: The value of bioluminescence imaging (BLI) for experimental cancer models has become firmly established. We applied BLI to the GL261 glioma model in the context of dendritic cell (DC) immunotherapy. Initial validation revealed robust linear correlations between in vivo, ex vivo and in vitro luciferase activity measurements. Ex vivo BLI demonstrated midline crossing and leakage of tumor cells. Orthotopically challenged mice followed with BLI showed an initial adaptation phase, after which imaging data correlated linearly with stereologically determined tumor dimensions. Transition from healthy to moribund state corresponded with an increasing in vivo flux but the onset of neurological deficit was clearly delayed compared to the onset of in vivo flux increase. BLI was implemented in prophylactic immunotherapy and imaging data were prognostic for therapy outcome. Three distinct response patterns were detected. Our data underscore the feasibility of in vivo BLI in an experimental immunotherapeutic setting in the GL261 glioma model.
ISSN: 0167-594X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Virology and Gene Therapy
Research Group for Neurobiology and Gene Therapy
Pediatric Hematology & Oncology Section (-)
Laboratory of Clinical Immunology
Nuclear Medicine & Molecular Imaging
Laboratory of Pediatric Immunology
× corresponding author
# (joint) last author

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