Title: The influence of yeast preoxygenation before brewery fermentation on yeast metabolism and the oxidative stress response
Authors: Verbelen, Pieter ×
Depraetere, Sofie
Winderickx, Joris
Delvaux, Freddy
Delvaux, Filip #
Issue Date: Mar-2009
Publisher: Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies
Series Title: FEMS Yeast Research vol:9 issue:2 pages:226-239
Article number: doi: 10.1111/j.1567-1364.2008.00476.x
Abstract: Yeast preoxygenation can offer important advantages to brewery fermentations by means of omitting the need to oxygenate the wort. However, the impact of yeast preoxygenation on yeast metabolism has never been assessed systematically. Therefore, expression analysis was performed of genes, which are of importance in oxygen-dependent pathways, oxidative stress response and general stress response during 8 hours of preoxygenation. The gene expression of the important transcription factors Hap1 and Rox1, involved in oxygen sensing, were both mainly increased in the first 3 hours, while YAP1 expression, which is involved in the oxidative stress response, increased drastically only in the first 45 minutes. The results also show that stress responsive genes (HSP12, SSA3, PAU5, SOD1, SOD2, CTA1 and CTT1) were induced during the process, together with the accumulation of trehalose. The accumulation of ergosterol and unsaturated fatty acids was accompanied by the expression of ERG1, ERG11 and OLE1. Genes involved in respiration (QCR9, COX15, CYC1, CYC7) also increased during preoxygenation. Yeast viability did not decrease during the process, and the fermentation performance of the yeast reached a maximum after 5 h of preoxygenation. These results suggest that yeast cells acquire a stress response along the preoxygenation period, which render them more resistant against the stressful conditions of the preoxygenation process and the subsequent fermentation
ISSN: 1567-1356
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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