Title: Single cell lineage analysis reveals mesenchymal multipotency of adult human periosteal cells
Authors: De Bari, Cosimo ×
Dell'accio, Francesco
Vanlauwe, Johan
Eyckmans, Jeroen
Khan, Ilyas
Archer, Charles W.
Jones, Elena
Mcgonagle, Dennis
Mitsiadis, Thimios
Pitzalis, Constantino
Luyten, Frank #
Issue Date: Apr-2006
Publisher: John Wiley & Sons
Series Title: Arthritis and Rheumatism vol:54 issue:4 pages:1209-1221
Abstract: OBJECTIVE: To investigate whether periosteal cells from adult humans have
features of multipotent mesenchymal stem cells (MSCs) at the single-cell level.
METHODS: Cell populations were enzymatically released from the periosteum of the
proximal tibia obtained from adult human donors and then expanded in monolayer.
Single-cell-derived clonal populations were obtained by limiting dilution.
Culture-expanded periosteal cell populations were tested for their growth
potential and for expression of conventional markers of MSCs and were subjected
to in vitro assays to investigate their multilineage potential. To assess their
multipotency in vivo, periosteal cells were injected into a regenerating mouse
tibialis anterior muscle for skeletal myogenesis or were either seeded into an
osteoinductive matrix and implanted subcutaneously into nude mice for
osteogenesis or implanted in a joint surface defect under a periosteal flap into
goats for chondrogenesis. Cell phenotypes were analyzed by histochemistry and
immunohistochemistry and by reverse transcription-polymerase chain reaction for
the expression of lineage-related marker genes. RESULTS: Regardless of donor age,
periosteal cells were clonogenic and could be expanded extensively in monolayer,
maintaining linear growth curves over at least 30 population doublings. They
displayed long telomeres and expressed markers of MSCs. Under specific
conditions, both parental and single-cell-derived clonal cell populations
differentiated to the chondrocyte, osteoblast, adipocyte, and skeletal myocyte
lineages in vitro and in vivo. CONCLUSION: Our study demonstrates that,
regardless of donor age, the adult human periosteum contains cells that, upon
enzymatic release and culture expansion, are multipotent MSCs at the single-cell
ISSN: 0004-3591
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Orthopaedics Section (-)
× corresponding author
# (joint) last author

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