Fertility patients are in a stressful situation at the time of clinical examination. That condition of stress influences hormonal levels, especially prolactin levels, in blood plasma and in seminal plasma. After masturbation previously stress-induced hyperprolactinemia levels are reduced. This is probably due to the decrease of stressful tension during the period of clinical consultation and not likely due to the relaxation by masturbation. In conclusion, the stress condition of fertility patients must be taken into account while evaluating hormonal data.