Title: Clonal proliferation of T-Cell large granular lymphocytes
Authors: Boeckx, Nancy ×
Uyttebroeck, Anne
Langerak, Anton W
Brusselmans, Caroline
Goossens, Willy
Bossuyt, Xavier #
Issue Date: Mar-2004
Publisher: Wiley-Liss
Series Title: Pediatric Blood & Cancer vol:42 issue:3 pages:275-277
Abstract: BACKGROUND: T-cell large granular lymphocyte (T-LGL) leukemia is a disorder only rarely reported in children. We diagnosed a new case of a clonal T-LGL proliferation in a 6-year-old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA). PROCEDURE: Flow cytometric analysis including TCR-Vbeta repertoire analysis and molecular studies using reverse transcriptase polymerase chain reaction (RT-PCR), PCR heteroduplex analysis and GeneScan analysis were performed to investigate the clonal nature of the T-LGL. RESULTS: Flow cytometric analysis revealed a Vbeta3 clonal nature. Molecular studies identified a clonal Vbeta3-Cbeta RT-PCR product. Both PCR heteroduplex analysis and GeneScan analysis found clonal TCRB and TCRG gene rearrangements. CONCLUSIONS: An underlying T-LGL leukemia should be investigated in the diagnostic evaluation of acquired PRCA and neutropenia in young children. Both flow cytometric and molecular analyses can be used to establish the clonal nature of T-LGL.
ISSN: 1545-5009
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Section Child - Miscellaneous (-)
Experimental Laboratory Immunology
Department of Oncology - miscellaneous
× corresponding author
# (joint) last author

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