European Respiratory Journal vol:32 issue:5 pages:1184-1194
We evaluated if a system of co-cultures of relevant cells [pneumocytes (A549), macrophages (THP-1), mast cells (HMC-1) and endothelial cells (EAHY926)] mimics responses to PM10 previously reported in vivo. The role of mast cells was considered of special interest.Single cultures, BICULTURES (A549 + HMC-1 @ 10:1 ratio; THP-1 + HMC-1 @ 2:1 ratio) and TRICULTURES (A549 + THP-1 + HMC-1 @ 10:2:1 ratio) were exposed to urban PM10 (24 h @ 0, 10, 30 or 100 microg.cm(-2)). In further experiments, EAHY926 cells were introduced in inserts above the TRICULTURES. The released cytokines were evaluated with a FACS array system.THP-1 + HMC-1 BICULTURES and the TRICULTURES released more G-CSF, MIP-1beta, IL-1beta, IL-8, IL-6, TNFalpha, and MIP-1alpha in response to PM10 than the sum of the single cultures. TRICULTURES + EAHY926 released more G-CSF, MIP-1alpha, IL-8 and MIP-1beta than the EAHY926 single culture.The BICULTURES, TRICULTURES and TRICULTURES + EAHY926, provide results that are consistent with the local and systemic effects previously described for PM effects, i.e. inflammation, endothelial dysfunction and bone marrow cell mobilization. Mast cells seem to play a significant role in the co-cultures responses.