Title: Co-cultures of multiple cell types mimic pulmonary cell communication in response to urban PM10
Authors: Alfaro-Moreno, E ×
Nawrot, Tim
Vanaudenaerde, Bart
Hoylaerts, Marc
Vanoirbeek, Jeroen
Nemery, Benoit
Hoet, Peter #
Issue Date: Nov-2008
Publisher: Published jointly by the Society and Munksgaard
Series Title: European Respiratory Journal vol:32 issue:5 pages:1184-1194
Abstract: We evaluated if a system of co-cultures of relevant cells [pneumocytes (A549), macrophages (THP-1), mast cells (HMC-1) and endothelial cells (EAHY926)] mimics responses to PM10 previously reported in vivo. The role of mast cells was considered of special interest.Single cultures, BICULTURES (A549 + HMC-1 @ 10:1 ratio; THP-1 + HMC-1 @ 2:1 ratio) and TRICULTURES (A549 + THP-1 + HMC-1 @ 10:2:1 ratio) were exposed to urban PM10 (24 h @ 0, 10, 30 or 100 In further experiments, EAHY926 cells were introduced in inserts above the TRICULTURES. The released cytokines were evaluated with a FACS array system.THP-1 + HMC-1 BICULTURES and the TRICULTURES released more G-CSF, MIP-1beta, IL-1beta, IL-8, IL-6, TNFalpha, and MIP-1alpha in response to PM10 than the sum of the single cultures. TRICULTURES + EAHY926 released more G-CSF, MIP-1alpha, IL-8 and MIP-1beta than the EAHY926 single culture.The BICULTURES, TRICULTURES and TRICULTURES + EAHY926, provide results that are consistent with the local and systemic effects previously described for PM effects, i.e. inflammation, endothelial dysfunction and bone marrow cell mobilization. Mast cells seem to play a significant role in the co-cultures responses.
ISSN: 0903-1936
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Occupational, Environmental and Insurance Medicine (-)
Environment and Health - miscellaneous
× corresponding author
# (joint) last author

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