Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast

Aerts, A; Zabrocki, Piotr; Govaert, Gilmer; Mathys, Janick; Carmona-Gutierrez, D; Madeo, F; Winderickx, Joris; Cammue, Bruno; Thevissen, Karin

doi:10.1016/j.febslet.2008.11.028

Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast

Author:

Aerts, A

Zabrocki, Piotr ; Govaert, Gilmer ; Mathys, Janick ; Carmona-Gutierrez, D ; Madeo, F ; Winderickx, Joris ; Cammue, Bruno ; Thevissen, Karin

Keywords:

LESEC, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Cell Biology, Apoptosis, Chronological lifespan, Microarray analysis, Mitochondrial function, Mitochondrial morphology, Respiration, SACCHAROMYCES-CEREVISIAE, GENE-EXPRESSION, INCREASED RESPIRATION, MUTANTS, IDENTIFICATION, INTERMEDIATE, DNA, DNA Transposable Elements, Gene Expression, Hydrogen Peroxide, Mitochondria, Protein Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, 0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology, 0603 Evolutionary Biology, 3101 Biochemistry and cell biology

Abstract:

We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological aging as compared to WT. Expression of genes encoding subunits of mitochondrial electron transport chain and ATP synthase is significantly downregulated in HsTnII, and as a consequence, HsTnII is not able to respire ethanol. All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis.