Title: Identification, purification and characterization of an acetoacetyl-CoA thiolase from rat liver peroxisomes
Authors: Antonenkov, VD
Croes, K
Waelkens, Etienne
Van Veldhoven, Paul P
Mannaerts, Guy # ×
Issue Date: May-2000
Publisher: Blackwell science ltd
Series Title: European Journal of Biochemistry vol:267 issue:10 pages:2981-2990
Abstract: Acetoacetyl-CoA specific thiolases catalyse the cleavage of acetoacetyl-CoA into two molecules of acetyl-CoA and the synthesis (reverse reaction) of acetoacetyl-CoA. The formation of acetoacetyl-CoA is the first step in cholesterol and ketone body synthesis. In this report we describe the identification of a novel acetoacetyl-CoA thiolase and its purification from isolated rat liver peroxisomes by column chromatography. The enzyme, which is a homotetramer with a subunit molecular mass of 42 kDa, could be distinguished from the cytosolic and mitochondrial acetoacetyl-CoA thiolases by its chromatographic behaviour, kinetic characteristics and partial internal amino-acid sequences. The enzyme did not catalyse the cleavage of medium or long chain 3-oxoacyl-CoAs. The enzyme cross-reacted with polyclonal antibodies raised against cytosolic acetoacetyl-CoA thiolase. The latter property was exploited to confirm the peroxisomal localization of the novel thiolase in subcellular fractionation experiments.
ISSN: 0014-2956
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Laboratory of Protein Phosphorylation and Proteomics
Laboratory of Lipid Biochemistry and Protein Interactions
Laboratory of Phosphoproteomics (-)
× corresponding author
# (joint) last author

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