Title: Further characterization of rat dihydroceramide desaturase: Tissue distribution, subcellular localization, and substrate specificity
Authors: Causeret, C ×
Geeraert, L
Van der Hoeven, Gerd
Mannaerts, Guy
Van Veldhoven, Paul P #
Issue Date: Oct-2000
Publisher: American Oil Chemists' Society
Series Title: Lipids vol:35 issue:10 pages:1117-1125
Conference: - edition:- location:- date:-
Abstract: The introduction of the double bond in the sphingoid backbone of sphingolipids occurs at the level of dihydroceramide via an NADPH-dependent desaturase, as discovered in permeabilized rat hepatocytes. In the rat, the enzyme activity, which has now been further characterized, appeared to be mostly enriched in liver and Harderian gland. By means of subcellular fractionation of rat liver homogenates and density gradient separation of microsomal fractions, the desaturase was localized to the endoplasmic reticulum. Various detergents were inhibitory to the enzyme, and maximal activities were obtained in the presence of NADPH and when the substrate was complexed to albumin. In the presence of albumin, the chain length of the fatty acid of the truncated dihydroceramides hardly affected the activity. Finally, in view of a likely evolutionary relationship between desaturases and hydroxylases, the formation of hydroxylated intermediates was analyzed. No evidence for their presence was found under our assay conditions.
ISSN: 0024-4201
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Lipid Biochemistry and Protein Interactions
Laboratory of Biosignaling & Therapeutics
× corresponding author
# (joint) last author

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