Microarray-based discovery of highly expressed olfactory mucosal genes: potential roles in the various functions of the olfactory system
Genter, MB × Van Veldhoven, Paul P Jegga, AG Sakthivel, B Kong, S Stanley, K Witte, DP Ebert, CL Aronow, BJ #
Amer physiological soc
Physiological genomics vol:16 issue:1 pages:67-81
We sought to gain a global view of tissue- specific gene expression in the olfactory mucosa ( OM), the major site of neurogenesis and neuroregeneration in adult vertebrates, by examination of its overexpressed genes relative to that in 81 other developing and adult mouse tissues. We used a combination of statistical and fold- difference criteria to identify the top 269 cloned cDNAs from an array of 8,734 mouse cDNA elements on the Incyte Mouse GEM1 array. These clones, representing known and poorly characterized gene transcripts, were grouped according to their relative expression patterns across the other tissues and then further examined with respect to gene ontology categories. Approximately one- third of the 269 genes were also highly expressed in developing and/ or adult central nervous system tissues. Several of these have been suggested or demonstrated to play roles in neurogenesis, neuronal differentiation, and/ or neuronal migration, further suggesting that many of the unknown genes that share this expression pattern may play similar roles. Highly OM- specific genes included a palate, lung, and nasal epithelium carcinoma- associated gene ( Plunc); sphingosine phosphate lyase ( Sgpl1), and paraoxonase 1 ( Pon1). Cell- type- specific expression within OM was established using in situ hybridization for several representative expression pattern clusters. Using the ENSEMBL- assembled mouse genome and comparative genomics analyses to the human genome, we assigned many of the unknown expressed sequence tags ( ESTs) and poorly characterized genes to either novel or known gene products and provided predictive classification. Further exploration of this database will provide additional insights into genes and pathways critical for olfactory neurogenesis, neuronal differentiation, olfaction, and mucosal defense.