Title: Gas chromatographic determination of alkyl lysophospholipids after solid-phase extraction from cell culture media
Authors: Coene, J ×
Van den Eeckhout, E
Herdewijn, Piet
Sandra, P #
Issue Date: Jan-1993
Publisher: Elsevier science bv
Series Title: Journal of chromatography vol:612 issue:1 pages:21-6
Abstract: The gas chromatographic determination of 1-O-octadecyl-2-O-methyl-DL-glycero-3-phosphorylcholine (Et-18-OMe), an anti-invasive alkyl lysophospholipid, in cell culture media is described. Sample clean-up was performed by solid-phase extraction on a weak cation-exchange column of the CBA type (carboxylic acid). For quantitation, the structural analogue Et-16-OMe as the internal standard was used after derivatization with trimethylsilyl bromide. The described method was free of interferences in cell culture media. The overall precision for twenty determinations was 14.99%.
ISSN: 0378-4347
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science