Title: Characterization of impurities in sisomicin and netilmicin by liquid chromatography/mass spectrometry
Authors: Li, Bo
Van Schepdael, Ann
Hoogmartens, Jos
Adams, Erwin # ×
Issue Date: Nov-2008
Series Title: Rapid communications in mass spectrometry : RCM vol:22 issue:22 pages:3455-3471
Abstract: The characterization of unknown impurities present in netilmicin and sisomicin by liquid chromatography (LC) coupled with mass spectrometry (MS) is described. The volatile ion-pairing agent trifluoroacetic acid (TFA) was used for the retention of the main compounds and their impurities on a reversed-phase (RP) C(18) column, because they are highly hydrophilic and basic compounds. The method showed good separation between netilmicin and its four potential related substances prescribed in the European Pharmacopoeia, which were identified by comparison of their retention times with those of the reference substances. Furthermore, in total 16 unknown impurities in a netilmicin sample and six in a sisomicin sample with unknown identity were detected. The structures of the unknown compounds were deduced based on comparison of fragmentation patterns with those of the reference substances investigated in LC/MS(n) experiments by the use of electrospray ion trap mass spectrometry. Copyright (c) 2008 John Wiley & Sons, Ltd.
ISSN: 0951-4198
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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