Title: Isolation and identification of two isomeric trihydroxy octadecenoic acids with prostaglandin E-like activity from onion bulbs (Allium cepa)
Authors: Ustünes, L ×
Claeys, M
Laekeman, Gert
Herman, A G
Vlietinck, A J
Ozer, A #
Issue Date: May-1985
Publisher: Elsevier Science
Series Title: Prostaglandins vol:29 issue:5 pages:847-65
Abstract: Two fractions with prostaglandin E-like activity were isolated from onion (Allium cepa) by using XAD-2 adsorption, silicic acid column chromatography and thin layer chromatography. The fractions were analyzed by gas chromatography/mass spectrometry and were characterized as isomeric mixtures of 9,10,13-trihydroxy-11-octadecenoic and 9,12,13-trihydroxy-10-octadecenoic acid, which are lipoxygenase metabolites of linoleic acid. Bio-assay, for which cascade superfusion was used and the rabbit coeliac and mesenteric arteries and the rat fundus strip were employed as assay organs, was utilized to monitor the bio-active profile throughout the isolation procedures. The activity of 1 microgram of the pharmacologically active fractions T1 and T2 was found to be equivalent to that of respectively 1.33 and 0.63 ng of prostaglandin E2.
ISSN: 0090-6980
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Centre for Pharmaceutical Care and Pharmaco-economics (-)
× corresponding author
# (joint) last author

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