Title: Structure of an RNase-related protein from Calystegia sepium
Authors: Rabijns, Anja ×
Verboven, Christel
Rougé, P
Barre, A
Van Damme, E J M
Peumans, W J
De Ranter, Camiel #
Issue Date: Apr-2002
Series Title: Acta crystallographica. Section D, Biological crystallography vol:58 issue:Pt 4 pages:627-33
Abstract: The structure of a catalytically inactive RNase-related protein from Calystegia sepium (CalsepRRP) has been resolved by protein crystallography at a resolution of 2.05 A and an R factor of 20.74%. Although the protein is completely devoid of ribonuclease activity, it adopts the typical alpha + beta structure of non-base-specific RNases. Analysis of the structure revealed that two amino-acid substitutions in the 'active' P1 site, in combination with the less hydrophobic/aromatic character of the B1 base-recognition site and a completely disrupted B2 base-recognition site, might account for this complete lack of activity.
ISSN: 1399-0047
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
× corresponding author
# (joint) last author

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