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Title: Enzymatic incorporation in DNA of 1,5-anhydrohexitol nucleotides
Authors: Vastmans, Karen ×
Pochet, Sylvie
Peys, A
Kerremans, Lucien
Van Aerschot, Arthur
Hendrix, Christel
Marlière, Philippe
Herdewijn, Piet #
Issue Date: Oct-2000
Series Title: Biochemistry vol:39 issue:42 pages:12757-12765
Abstract: The ability of several DNA polymerases to catalyze the template-directed synthesis of duplex oligonucleotides containing a base pair between a nucleotide with anhydrohexitol ring and its natural complement has been investigated. All DNA polymerases were able to accept the chemically synthesized anhydrohexitol triphosphate as substrate and to catalyze the incorporation of one anhydrohexitol nucleotide. However, only family B DNA polymerases succeeded in elongating the primer after the incorporation of an anhydrohexitol nucleotide. In this family, Vent (exo(-)) DNA polymerase is the most successful one and was therefore selected for further investigation. Results revealed that at high enzyme concentrations six hATPs could be incorporated; however, a selective incorporation proved only feasible under experimental conditions where no more than two analogues could be inserted. Also the synthesis of a mixed HNA-DNA sequence was examined. Kinetic parameters for incorporation of one anhydrohexitol adenine nucleoside were similar to those of its natural analogue.
ISSN: 0006-2960
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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