Title: Mass spectrometric study to characterize thioisoindole derivatives of aminoglycoside antibiotics
Authors: Kaale, Eliangiringa ×
Govaerts, Cindy
Hoogmartens, Jos
Van Schepdael, Ann #
Issue Date: 2005
Series Title: Rapid communications in mass spectrometry vol:19 issue:20 pages:2918-2922
Abstract: Aminoglycoside antibiotics are widely used to treat serious Gram-negative and Gram-positive bacterial infections. The lack of a UV chromophore presents a problem in the analysis of aminoglycosides. Derivatization with 1,2-phthalic dicarboxaldehyde (OPA) in the presence of a thiol made it possible to introduce a UV chromophoric thioisoindole moiety. A qualitative mass spectrometry study was carried out to confirm the molecular identity of the products formed. The conditions described earlier to derivatize gentamicin and kanamycin yielded products in which all primary amino groups are fully derivatized. On the other hand, with tobramycin and amikacin, there was also formation of incompletely derivatized products that contained one thioisoindole group less than the fully derivatized product. This study has therefore brought an additional insight into the nature of the OPA-aminoglycoside derivatives studied.
ISSN: 0951-4198
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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