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Title: Assay and purity control of oxytetracycline and doxycycline by thin-layer chromatography--a comparison with liquid chromatography
Authors: Weng Naidong ×
Geelen, S
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Jan-1990
Series Title: Journal of pharmaceutical and biomedical analysis vol:8 issue:8-12 pages:891-8
Abstract: A thin-layer chromatographic (TLC) method using densitometry is described for the assay and purity control of oxytetracycline and doxycycline. With a mobile phase of dichloromethane-methanol-water (59:35:6, v/v/v) and a silica gel thin-layer, previously sprayed with 10% sodium edetate solution adjusted to pH 9.0, all the potential impurities of oxytetracycline or doxycycline are well separated from the main components and from each other. Results obtained with TLC are compared with those obtained by previously established liquid chromatography (LC) methods using poly(styrene-divinylbenzene) stationary phases. A good correlation was obtained (r greater than 0.9999). For TLC the relative standard deviation (RSD) for the assay of the main component was less than 2%, for LC the RSD was less than 1%.
ISSN: 0731-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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