Title: Involvement of glycan chains in the antigenicity of Rapana thomasiana hemocyanin
Authors: Siddiqui, Nurul Islam
Idakieva, Krassimira
Demarsin, B
Doumanova, Lyubka
Compernolle, Frans
Gielens, Constant # ×
Issue Date: 2007
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:361 issue:3 pages:705-711
Abstract: Functional unitFunctional unit (FU) RtH2-e from Rapana thomasiana hemocyanin (Hc) was degraded into small fragments with chymotrypsin. The glycopeptides were separated from the non-glycosylated peptides by chromatography on Concanavalin-A–Sepharose and characterized by mass spectrometry. The glycan part of the glycopeptides (all with common peptide stretch of 14 amino acids) consists of the classical trimannosyl-N,N-diacetylchitobiose core for N-glycosylation, predominantly extended with a unique tetrasaccharide that is branched on fucose. In inhibition ELISA experiments, the glycopeptides interfered in the complex formation between FU RtH2-e and rabbit antibodies against Rapana Hc (about 30% of inhibition). The inhibition also was retained after treatment of the glycopeptides with pronase in order to completely destroy the peptide part. The inhibitory effect of the non-glycosylated peptides, on the other hand, was very low. This study thus demonstrates that the glycans attached to FU RtH2-e contribute to the antigenicity of Rapana Hc.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
Chemistry - miscellaneous
× corresponding author
# (joint) last author

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