Title: Noninvasive and quantitative monitoring of adult neuronal stem cell migration in mouse brain using bioluminescence imaging
Authors: Reumers, Veerle
Deroose, Christophe
Krylychkina, Olga
Nuyts, Johan
Geraerts, Martine
Mortelmans, Luc
Gijsbers, Rik
Van Den Haute, Chris
Debyser, Zeger
Baekelandt, Veerle # ×
Issue Date: Sep-2008
Publisher: AlphaMed Press
Series Title: Stem Cells vol:26 issue:9 pages:2382-2390
Abstract: It is now generally accepted that continuous neurogenesis occurs in the adult mammalian brain, including that of humans. Modulation of adult neurogenesis can provide therapeutic benefits for various brain disorders, including stroke and Parkinson's disease. The subventricular zone-olfactory bulb pathway is one of the preferred model systems by which to study neural stem cell proliferation, migration, and differentiation in adult rodent brain. Research on adult neurogenesis would greatly benefit from reliable methods for long-term noninvasive in vivo monitoring. We have used lentiviral vectors encoding firefly luciferase to stably mark endogenous neural stem cells in the mouse subventricular zone. We show that bioluminescence imaging (BLI) allows quantitative follow-up of the migration of adult neural stem cells into the olfactory bulb in time. Moreover, we propose a model to fit the kinetic data that allows estimation of migration and survival times of the neural stem cells using in vivo BLI. Long-term expression of brain-derived neurotrophic factor in the subventricular zone attenuated neurogenesis, as detected by histology and BLI. In vivo monitoring of the impact of drugs or genes on adult neurogenesis is now within reach.
ISSN: 1066-5099
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group for Neurobiology and Gene Therapy
Nuclear Medicine & Molecular Imaging
Interdepartemental Stem Cell Institute (-)
Molecular Virology and Gene Therapy
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
21 Reumers SC 2008.pdf Published 2520KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science