Title: Nitric oxide enhances Oct-4 expression in bone marrow stem cells and promotes endothelial differentiation
Authors: Chu, Ling ×
Jiang, Yuehua
Hao, Hong
Xia, Yong
Xu, Jian
Liu, Zehao
Verfaillie, Catherine
Zweier, Jay L
Liu, Zhenguo #
Issue Date: Sep-2008
Series Title: European Journal of Pharmacology vol:591 issue:1-3 pages:59-65
Abstract: This study was designed to investigate the role of nitric oxide (NO) in bone marrow stem cells and their differentiation into endothelial cells in vitro. Adult mouse bone marrow multipotent progenitor cells (MAPCs) were used as the source of stem cells. Oct-4 expression (both mRNA and protein) was significantly increased by up to 68.0% in MAPCs when incubated with NO donors DETA-NONOate or sodium nitroprusside (SNP) in a concentration-dependant manner (n=3, P<0.05). However, the cell proliferation was dramatically decreased by over 3-folds when treated with DETA-NONOate or SNP for 48 h (n=3, P<0.05). When MAPCs were exposed to DETA-NONOate (100 microM) for the first 48 h during differentiation, the expression (both mRNA and protein) of vWF was significantly increased at day 14 in the differentiating cells. The effects of DETA-NONOate or SNP on cell proliferation, Oct-4 expression and endothelial differentiation of MAPCs were not affected by the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one or cGMP analog 8-Br-cGMP. These data indicate that NO may regulate both the pluripotency and differentiation of MAPCs via a cGMP-independent mechanism.
ISSN: 0014-2999
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdepartemental Stem Cell Institute (-)
Department of Materials Engineering - miscellaneous
× corresponding author
# (joint) last author

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