Title: Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity
Authors: Ulloa-Montoya, Fernando ×
Kidder, Benjamin L
Pauwelyn, Karen A
Chase, Lucas G
Luttun, Aernout
Crabbe, Annelies
Geraerts, Martine
Sharov, Alexei A
Piao, Yulan
Ko, Minoru S H
Hu, Wei-Shou
Verfaillie, Catherine #
Issue Date: 2007
Publisher: BioMed Central Ltd.
Series Title: Genome Biology vol:8 issue:8
Article number: R163
Abstract: BACKGROUND: Recently, several populations of postnatal stem cells, such as multipotent adult progenitor cells (MAPCs), have been described that have broader differentiation ability than classical adult stem cells. Here we compare the transcriptome of pluripotent embryonic stem cells (ESCs), MAPCs, and lineage-restricted mesenchymal stem cells (MSCs) to determine their relationship. RESULTS: Applying principal component analysis, non-negative matrix factorization and k-means clustering algorithms to the gene-expression data, we identified a unique gene-expression profile for MAPCs. Apart from the ESC-specific transcription factor Oct4 and other ESC transcripts, some of them associated with maintaining ESC pluripotency, MAPCs also express transcripts characteristic of early endoderm and mesoderm. MAPCs do not, however, express Nanog or Sox2, two other key transcription factors involved in maintaining ESC properties. This unique molecular signature was seen irrespective of the microarray platform used and was very similar for both mouse and rat MAPCs. As MSC-like cells isolated under MAPC conditions are virtually identical to MSCs, and MSCs cultured in MAPC conditions do not upregulate MAPC-expressed transcripts, the MAPC signature is cell-type specific and not merely the result of differing culture conditions. CONCLUSION: Multivariate analysis techniques clustered stem cells on the basis of their expressed gene profile, and the genes determining this clustering reflected the stem cells' differentiation potential in vitro. This comparative transcriptome analysis should significantly aid the isolation and culture of MAPCs and MAPC-like cells, and form the basis for studies to gain insights into genes that confer on these cells their greater developmental potency.
ISSN: 1465-6906
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
17 Ulloa GB 2007.pdfMain article Published 735KbAdobe PDFView/Open
Ulloa Montoya - Genome Biology - 2007.pdf Published 698KbAdobe PDFView/Open


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science