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Title: Modeling lymphangiogenesis in a three-dimensional culture system
Authors: Bruyère, Françoise
Melen-Lamalle, Laurence
Blacher, Silvia
Roland, Guy
Thiry, Marc
Moons, Lieve
Frankenne, Francis
Carmeliet, Peter
Alitalo, Kari
Libert, Claude
Sleeman, Jonathan P
Foidart, Jean-Michel
Noël, Agnès # ×
Issue Date: May-2008
Publisher: Nature Pub. Group
Series Title: Nature Methods vol:5 issue:5 pages:431-437
Abstract: A lack of appropriate in vitro models of three-dimensional lymph vessel growth hampers the study of lymphangiogenesis. We developed a lymphatic ring assay--a potent, reproducible and quantifiable three-dimensional culture system for lymphatic endothelial cells that reproduces spreading of endothelial cells from a pre-existing vessel, cell proliferation, migration and differentiation into capillaries. In the assay, mouse thoracic duct fragments are embedded in a collagen gel, leading to the formation of lumen-containing lymphatic capillaries, which we assessed by electron microscopy and immunostaining. We developed a computerized method to quantify the lymphatic network. By applying this model to gene-deficient mice, we found evidence for involvement of the matrix metalloproteinase, MMP-2, in lymphangiogenesis. The lymphatic ring assay bridges the gap between two-dimensional in vitro models and in vivo models of lymphangiogenesis, can be used to exploit the potential of existing transgenic mouse models, and rapidly identify regulators of lymphangiogenesis.
URI: 
ISSN: 1548-7091
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Vesalius Research Centre (-)
Animal Physiology and Neurobiology Section - miscellaneous
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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