Title: Extraction and liquid chromatographic method for the determination of simmondsin in plasma
Authors: Van Boven, Maurits ×
Daenens, Paul
Cokelaere, Marnix
Decuypere, Eddy #
Issue Date: May-1994
Series Title: Journal of chromatography. B, Biomedical applications vol:655 issue:2 pages:281-5
Abstract: A solid-phase method for the extraction of simmondsin from plasma was developed along with a liquid chromatographic method for the quantitative measurement of this compound. The extraction of simmondsin is realised with activated carbon. Chromatography is performed on a 25 x 0.46 cm I.D. RP-18, 5-micron HPLC column with a water-methanol (85:15, v/v) mobile phase and ultraviolet absorbance detection at 217 nm. The limit of detection is 100 ng (using 1 ml of plasma). The linear quantitation range is 0.1-200 micrograms/ml.
ISSN: 0378-4347
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdisciplinary Research Facility Life Sciences, Campus Kulak Kortrijk
Division of Livestock-Nutrition-Quality (-)
Toxicology and Pharmacology
× corresponding author
# (joint) last author

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