Title: His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases
Authors: Rombouts, Sigrid ×
Fierens, Ellen
Vandermarliere, Elien
Voet, Arnout
Gebruers, Kurt
Beaugrand, Johnny
Courtin, Christophe
Delcour, Jan
De Maeyer, Marc
Rabijns, Anja
Van Campenhout, Steven
Volckaert, Guido #
Issue Date: 2009
Series Title: Journal of enzyme inhibition and medicinal chemistry vol:24 issue:1 pages:38-46
Abstract: Recently, a novel wheat thaumatin-like protein, TLXI, which inhibits microbial glycoside hydrolase family (GH) 11 xylanases has been identified. It is the first xylanase inhibitor that exerts its inhibition in a non-competitive way. In the present study we gained insight into the interaction between TLXI and xylanases via combined molecular modeling and mutagenic approaches. More specifically, site-specific mutation of His22, situated on a loop which distinguishes TLXI from other, non-inhibiting, thaumatin-like proteins, and subsequent expression of the mutant in Pichia pastoris resulted in a protein lacking inhibition capacity. The mutant protein was unable to form a complex with GH11 xylanases. Based on these findings, the interaction of TLXI with GH11 xylanases is discussed.
ISSN: 1475-6366
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
Centre for Food and Microbial Technology
Faculty of Pharmaceutical Sciences - miscellaneous
Division of Gene Technology (-)
× corresponding author
# (joint) last author

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