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Title: Determination of capsaicinoids in topical cream by liquid-liquid extraction and liquid chromatography
Authors: Kaale, Eliangiringa ×
Van Schepdael, Ann
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Nov-2002
Series Title: Journal of pharmaceutical and biomedical analysis vol:30 issue:4 pages:1331-7
Abstract: A reversed-phase liquid chromatography (LC) method has been developed, optimised and validated for the separation and quantitation of capsaicin (CP) and dihydrocapsaicin (DHCP) in a topical cream formulation. Sample preparation involves liquid-liquid extraction prior to LC analysis. The method uses a Hypersil C(18) BDS, 5 micrometer, 250x4.6 mm I.D. column maintained at 35 degrees C. The mobile phase comprises methanol, water, acetonitrile (ACN) and acetic acid (47:42:10:1, v/v/v/v) at a flow rate of 1.0 ml/min. Robustness was evaluated by performing a central composite face-centred design (CCF) experiment. The method shows good selectivity, linearity, sensitivity and repeatability. The conditions allow the separation and quantitation of CP and DHCP without interference from the other substances contained in the cream.
ISSN: 0731-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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