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Title: Kinetic study of CYP3A4 activity on verapamil by capillary electrophoresis
Authors: Zhang, Jie ×
Thi Thanh Ha, Pham
Lou, Yijia
Hoogmartens, Jos
Van Schepdael, Ann #
Issue Date: Sep-2005
Series Title: Journal of pharmaceutical and biomedical analysis vol:39 issue:3-4 pages:612-7
Abstract: The use of capillary electrophoresis (CE) for the determination of CYP3A4 activity with verapamil as a substrate was investigated. CYP3A4 activity was determined by the quantitation of the product, norverapamil, based on separation by CE. The separation conditions were as follows: capillary, 80.5 cm (75 microm i.d., 72 cm effective length); 50 mM sodium phosphate buffer (pH 8.8); 20 kV (100 microA) applied voltage; UV detection at 200 nm; capillary temperature, 25 degrees C. With the developed CYP3A4 activity assay and the Lineweaver-Burk equation, the Michaelis-Menten parameters Km and Vmax for formation of norverapamil from verapamil in the presence of CYP3A4 were determined and were 22.8+/-2.5 microM and 7.67+/-0.26 pmol/min/pmol (or 983 pmol/min/mg) CYP3A4, respectively.
ISSN: 0731-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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