Title: Determination of the composition of gentamicin sulfates by 1H and 13C nuclear magnetic spectroscopy
Authors: Busson, Roger
Claes, Paul
Vanderhaeghe, Hubert # ×
Issue Date: Jul-1986
Publisher: Aoac international
Series Title: Journal - Association of Official Analytical Chemists vol:69 issue:4 pages:601-608
Abstract: The British Pharmacopoeia test controlling the composition of gentamicin sulfate is based on CW 60 MHz magnetic resonance spectroscopy. Application of this method to FT 90 MHz spectra was evaluated. Results clearly show the limitations of this technique and point out the need for more reliable assay methods. Thus a 13C nuclear magnetic resonance (NMR) procedure for quantitative analysis of gentamicin sulfate was developed. Ratios of 4 gentamicin components (C1, C2, C1a, and C2a) were obtained from peak height measurements of selected resonance signals in spectra recorded under steady-state conditions. Relative response factors were determined from spectra of a reference mixture or, alternatively, from spectra of the individual pure components. Results obtained by the 13C NMR method were in agreement with those obtained by liquid chromatography using pre-column derivatization.
ISSN: 0004-5756
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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