Title: Crystallization and preliminary X-ray diffraction analysis of a novel pectate lyase from Azospirillum irakense
Authors: Novoa de Armas, Hector ×
Rabijns, Anja
Verboven, Christel
Desair, Josephus
Vande Broek, Ann
Vanderleyden, Jozef
De Ranter, Camiel #
Issue Date: Feb-2002
Series Title: Acta crystallographica. Section D, Biological crystallography vol:58 issue:Pt 2 pages:292-5
Abstract: The PelA gene from the N(2)-fixing plant-associated bacterium Azospirillum irakense encodes a pectate lyase. Analysis of the corresponding amino-acid sequence revealed no homology to other bacterial, plant and fungal pectinases of known published structure, resulting in the classification of the enzyme in a new pectate lyase family. The A. irakense PelA has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are hexagonal, with unit-cell parameters a = b = 85.55, c = 230.13 A, gamma = 120 degrees, and belong to space group P6(5)22 or P6(1)22, having one molecule per asymmetric unit. Diffraction data to a resolution of 1.97 A were collected at synchrotron facilities, as well as a three-wavelength MAD data set from an Hg-derivative crystal to a resolution of 2.6 A.
ISSN: 1399-0047
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science