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Title: Capillary electrophoresis analysis of gentamicin sulphate with UV detection after pre-capillary derivatization with 1,2-phthalic dicarboxaldehyde and mercaptoacetic acid
Authors: Kaale, Eliangiringa ×
Leonard, Stefanie
Van Schepdael, Ann
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Oct-2000
Series Title: Journal of Chromatography A vol:895 issue:1-2 pages:67-79
Abstract: A selective, sensitive, and rapid pre-capillary derivatization method for determination of the multicomponent aminoglycoside antibiotic gentamicin is described. The derivatization reagents 1,2-phthalic dicarboxaldehyde and mercaptoacetic acid were used and the thioisoindole derivative was UV detected at 330 nm. A central composite experimental design was performed to optimize selectivity and derivatization conditions. Baseline separation of gentamicin C1, C1a, C2, C2a, C2b, sisomicin and several minor components was achieved with a background electrolyte containing 30 mM sodium tetraborate, 7.5 mM beta-cyclodextrin and 12.5% (v/v) methanol at pH 10. Quantitative analysis was performed and illustrated the potential use of capillary electrophoresis for the identification and quantitation of gentamicin as an alternative to methods prescribed in the United States Pharmacopeia and European Pharmacopoeia.
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
Practical Training, Faculty of Pharmaceutical Sciences
× corresponding author
# (joint) last author

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