Title: Inhibition study of angiotensin converting enzyme by capillary electrophoresis after enzymatic reaction at capillary inlet
Authors: Van Dyck, Sigrid ×
Nováková, Sona
Van Schepdael, Ann
Hoogmartens, Jos #
Issue Date: Sep-2003
Series Title: Journal of Chromatography A vol:1013 issue:1-2 pages:149-56
Abstract: Capillary electrophoresis was used to study the inhibition of angiotensin-converting enzyme (ACE) by different inhibitors. Reaction occurred at the capillary inlet during a predetermined waiting period, followed by the electrophoretic separation of the reaction compounds. ACE activity was determined by the quantification of the reaction product, hippuric acid, at 230 nm. The technique was used to study the potency of five different inhibitors (captopril, lisinopril, perindoprilat, quinaprilat and benazeprilat). During a kinetic study, the Ki value of captopril was estimated to be 55.4 +/- 8.8 nM, a value consistent with previously reported values.
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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