Title: TRPP2 and TRPV4 form a polymodal sensory channel complex
Authors: Koetttgen, Michael ×
Buchholz, Bjoern
Garcia-Gonzalez, Miguel A
Kotsis, Fruzsina
Fu, Xiao
Doerken, Mara
Boehlke, Christopher
Steffl, Daniel
Tauber, Robert
Wegierski, Tomasz
Nitschke, Roland
Suzuki, Makoto
Kramer-Zucker, Albrecht
Germino, Gregory G
Watnick, Terry
Prenen, Jean
Nilius, Bernd
Kuehn, E. Wolfgang
Walz, Gerd #
Issue Date: Aug-2008
Publisher: Rockefeller Institute Press
Series Title: Journal of Cell Biology vol:182 issue:3 pages:437-447
Abstract: The primary cilium has evolved as a multifunctional cellular compartment that decorates most vertebrate cells. Cilia sense mechanical stimuli in various organs, but the molecular mechanisms that convert the deflection of cilia into intracellular calcium transients have remained elusive. Polycystin-2 (TRPP2), an ion channel mutated in polycystic kidney disease, is required for ciliamediated calcium transients but lacks mechanosensitive properties. We find here that TRPP2 utilizes TRPV4 to form a mechano- and thermosensitive molecular sensor in the cilium. Depletion of TRPV4 in renal epithelial cells abolishes flow-induced calcium transients, demonstrating that TRPV4, like TRPP2, is an essential component of the ciliary mechanosensor. Because TRPV4-deficient zebrafish and mice lack renal cysts, our findings challenge the concept that defective ciliary flow sensing constitutes the fundamental mechanism of cystogenesis.
ISSN: 0021-9525
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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