Title: HGF/SF and menthol increase human glioblastoma cell calcium and migration
Authors: Wondergem, Robert ×
Ecay, Tom W
Mahieu, Frank
Owsianik, Grzegorz
Nilius, Bernd #
Issue Date: Jul-2008
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:372 issue:1 pages:210-215
Abstract: This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase [Ca2+](i) needed for DBTRG cell migration. (C) 2008 Elsevier Inc. All rights reserved.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research (VIB-KU Leuven Center for Brain & Disease Research)
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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