Title: Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis
Authors: Behets, Jonas ×
Declerck, Priscilla
Delaedt, Yasmine
Verelst, Lieve
Ollevier, Frans #
Issue Date: Dec-2006
Series Title: Current microbiology vol:53 issue:6 pages:506-9
Abstract: Real-time polymerase chain reaction melting curve analysis (MCA) allows differentiation of several free-living amoebae species. Distinctive characteristics were found for Naegleria fowleri, N. lovaniensis, N. australiensis, N. gruberi, Hartmanella vermiformis, and Willaertia magna. Species specificity of the amplicons was confirmed using agarose gel electrophoresis and sequence-based approaches. Amplification efficiency ranged from 91% to 98%, indicating the quantitative potential of the assay. This MCA approach can be used for quantitative detection of free-living amoebae after cultivation but also as a culture-independent detection method.
ISSN: 0343-8651
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Ecology, Evolution and Biodiversity Conservation Section
Laboratory for Aquatic Ecology and Evolutionary Biology (-)
× corresponding author
# (joint) last author

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