Title: A fully functional deletion staphylokinase derivative crystallizes in two non-isomorphous monoclinic modifications
Authors: Rabijns, Anja ×
De Bondt, H L
Collen, Desire
De Ranter, Camiel #
Issue Date: Mar-1999
Series Title: Acta crystallographica. Section D, Biological crystallography vol:55 issue:Pt 3 pages:709-11
Abstract: Two non-isomorphous monoclinic types of diffraction-quality crystals of Delta10Sak, a fully functional staphylokinase derivative lacking the first ten amino acids, have been grown by the hanging-drop vapour-diffusion technique. Type I crystals grow from a solution containing Zn(OAc)2, Tris buffer pH 7.5 and PEG 8000, while type II crystals can be obtained from a solution containing MgCl2, Tris buffer pH 8.5 and PEG 4000. Both crystal types were suitable for data collection (to 2.4 and 2.6 A resolution, respectively) and further structural investigation.
ISSN: 2059-7983
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
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