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Title: Effects of maternal retinoic acid administration in a congenital diaphragmatic hernia rabbit model
Authors: Gallot, Denis ×
Coste, Karen
Jani, Jacques
Roubliova, Xenia
Marceau, Geoffroy
Velemir, Luka
Verheyen, An
Lemery, Didier
Sapin, Vincent
Deprest, Jan #
Issue Date: Jun-2008
Publisher: W.B. Saunders
Series Title: Pediatric pulmonology vol:43 issue:6 pages:594-603
Abstract: Maternal retinoid administration has beneficial effects on lung development in the nitrofen rodent toxic model of congenital diaphragmatic hernia (DH). We wanted to investigate the effects in a surgical model, where the retinoid signaling pathway is not primarily disrupted by the toxic agent. We created DH in fetal rabbits at day 23 of gestation, administrated to the does all trans-retinoic acid (ATRA) or vehicle (VHC) intramuscularly for 8 consecutive days and harvested normal and operated (DH) fetuses at 31 d (n = 7 in each group). Normal lungs exposed to ATRA had increased surfactant protein mRNA levels without change in type II pneumocyte density. There was no measurable effect on lung-to-body weight ratio and airway morphometry by ATRA. In DH lungs (DH/VHC) surfactant protein mRNA levels were increased, as well as the density of type II pneumocytes. When supplemented with ATRA (DH/ATRA) these parameters returned to normal (VHC). Cell proliferation or apoptosis were not influenced by ATRA supplementation. In conclusion, maternal ATRA supplementation does not affect gross anatomic, morphologic or proliferation indices in hypoplastic lungs related to surgically induced DH in rabbit. However, ATRA lowers surfactant protein expression and normalizes type I/II pneumocyte ratio to what is observed in normal lungs.
URI: 
ISSN: 8755-6863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Foetal Medicine Section (-)
Vesalius Research Centre (-)
Laboratory of Translational Genetics (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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