Title: Enrichment of collagen plugs with platelets and amniotic fluid cells increases cell proliferation in sealed iatrogenic membrane defects in the foetal rabbit model
Authors: Liekens, Daisy
Lewi, Liesbeth
Jani, Jacques
Heyns, Liesbeth
Poliard, Eline
Verbist, Godelieve
Ochsenbein-Kölble, Nicole
Hoylaerts, Marc
Deprest, Jan # ×
Issue Date: Jun-2008
Publisher: John Wiley & Sons
Series Title: Prenatal Diagnosis vol:28 issue:6 pages:503-507
Abstract: OBJECTIVES: The purpose of this study was to evaluate cell proliferation in platelet-enriched collagen plugs with and without addition of amniotic fluid-derived heterologous foetal cells to seal an iatrogenic membrane defect in the foetal rabbit model. METHODS: Amniotic fluid cells were harvested from three donor does at 23 days of gestation (term = 32 days) and labelled with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE). In 42 other does, foetal membrane defects were induced by foetoscopic needle puncture at 23 days of gestation, and closed with either a platelet-enriched collagen plug with (n = 44) or without (n = 32) amniotic fluid cells. At 30 days of gestation, the defects were harvested and assessed microscopically. RESULTS: The plugs enriched with heterologous amniotic fluid cells more commonly had proliferating cells in the centre of the plug than those without cell addition. CFDA-SE labelling confirmed the presence of heterologous amniotic fluid cells over the entire membrane plug. Cell typing showed a mixture of fibroblasts and epithelial cells at the wound edges, whereas in the centre, there was an abundance of fibroblasts. CONCLUSION: When sealing iatrogenic membrane defects in the foetal rabbit model, enrichment of collagen plugs with platelets and amniotic fluid-derived heterologous foetal cells increases local cell proliferation.
ISSN: 0197-3851
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Foetal Medicine Section (-)
Section Woman - Miscellaneous (-)
Molecular and Vascular Biology
× corresponding author
# (joint) last author

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