Title: Two new scorpion toxins that target voltage-gated Ca2+ and Na+ channels
Authors: Olamendi-Portugal, Timoteo ×
García, Blanca Inés
López-González, Ignacio
Van Der Walt, Jurg
Dyason, Karin
Ulens, Chris
Tytgat, Jan
Felix, Ricardo
Darszon, Alberto
Possani, Lourival D #
Issue Date: Dec-2002
Series Title: Biochemical and Biophysical Research Communications vol:299 issue:4 pages:562-8
Abstract: This report describes the isolation, primary structure determination, and functional characterization of two similar toxins from the scorpion Parabuthus granulatus named kurtoxin-like I and II (KLI and KLII, respectively). KLII from P. granulatus is identical to kurtoxin from Parabuthus transvaalicus (a 63 amino-acid long toxin) whereas KLI is a new peptide containing 62 amino acid residues closely packed by four disulfide bridges with a molecular mass of 7244. Functional assays showed that both toxins, KLI and kurtoxin from P. granulatus, potently inhibit native voltage-gated T-type Ca(2+) channel activity in mouse male germ cells. In addition, KLI was shown to significantly affect the gating mechanisms of recombinant Na(+) channels and weakly block alpha(1)3.3Ca(V) channels expressed in Xenopus oocytes. KLI and kurtoxin from P. granulatus represent new probes to study the role of ion channels in germ cells, as well as in cardiac and neural tissue.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Toxicology and Pharmacology
Laboratory of Structural Neurobiology
× corresponding author
# (joint) last author

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