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Title: Liquid chromatographic method for analysis of saponins in Maesa balansae extract active against leishmaniasis
Authors: Leonard, Stefanie ×
Capote, Raul
Germonprez, Nils
Van Puyvelde, Luc
De Kimpe, Norbert
Vermeersch, Hans
Rosier, Jan
Maes, Louis
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Sep-2003
Series Title: Journal of Chromatography A vol:1012 issue:1 pages:39-46
Abstract: A liquid chromatographic method was developed for the separation of six related triterpenoid saponins in Maesa balansae extracts with different purity, active against leishmaniasis. As stationary phase a Hypersil BDS C18 column (3 microm), 100 x 4.6 mm was used. The mobile phase was a mixture of methanol, acetonitrile, 5% (m/v) ammonium acetate, pH 6.5 and water. A linear gradient was developed for the analysis of crude extracts. An isocratic method was developed to analyze purified samples that mainly contained saponins 3 and 4, the most active saponins. The isocratic LC method was optimized and the robustness was evaluated with an experimental design. The method showed good selectivity, repeatability, linearity and sensitivity.
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Practical Training, Faculty of Pharmaceutical Sciences
Pharmaceutical Analysis
× corresponding author
# (joint) last author

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