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Title: Reduced nonspecific adsorption on covalently immobilized protein surfaces using poly(ethylene oxide) containing blocking agents
Authors: Frederix, Filip ×
Bonroy, Kristien
Reekmans, Gunter
Laureyn, Wim
Campitelli, Andrew
Abramov, Mikhael A
Dehaen, Wim
Maes, Guido #
Issue Date: Jan-2004
Series Title: Journal of biochemical and biophysical methods vol:58 issue:1 pages:67-74
Abstract: In a number of applications, e.g. DNA/protein micro-array technology, enzyme-linked immunosorbent assay (ELISA) technology or surface plasmon resonance (SPR) technology, the covalent coupling of proteins to surfaces is required. Following the covalent coupling of proteins, the remaining reactive groups should be blocked in order to avoid covalent binding of the analyte to the reactive surface. To this end, preferably blocking agents containing groups that avoid nonspecific adsorption should be used. These blocking agents are typically ethanolamine and cysteine for protein coupling via amino groups and thiol groups, respectively. This report presents novel blocking agents containing poly(ethylene oxide) (PEO) groups. These blocking agents show enhanced qualities to avoid nonspecific adsorption and can therefore have advantages in versatile protein-surface technologies.
ISSN: 0165-022X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Pharmaceutical Sciences - miscellaneous
Molecular Design and Synthesis
Quantum Chemistry and Physical Chemistry Section
× corresponding author
# (joint) last author

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