Title: Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 Cells investigated by raster-scanning image correlation spectroscopy (RICS)
Authors: Gielen, Ellen
Smisdom, Nick
De Clercq, Ben
Vandeven, Martin
Gijsbers, Rik
Debyser, Zeger
Rigo, Jean-Michel
Hofkens, Johan
Engelborghs, Yves
Ameloot, Marcel # ×
Issue Date: Sep-2008
Publisher: Kluwer Academic/Plenum Publishers
Series Title: Journal of Fluorescence vol:18 issue:5 pages:813-819
Abstract: Many membrane proteins and lipids are partially confined in substructures ranging from tens of nanometers to micrometers in size. Evidence for heterogeneities in the membrane of oligodendrocytes, i.e. the myelin-producing cells of the central nervous system, is almost exclusively based on detergent methods. However, as application of detergents can alter the membrane phase behaviour, it is important to investigate membrane heterogeneities in living cells. Here, we report on the first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique. We implemented RICS on a standard confocal laser-scanning microscope with one-photon excitation and analog detection. Measurements on FITC-dextran were used to evaluate the performance of the system and the data analysis procedure.
ISSN: 1053-0509
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Virology and Gene Therapy
Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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