Title: Comparative peptidomics of Caenorhabditis elegans versus C. briggsae by LC-MALDI-TOF MS
Authors: Husson, Steven ×
Landuyt, Bart
Nys, Thomas
Baggerman, Geert
Boonen, Kurt
Clynen, Elke
Lindemans, Marleen
Janssen, Tom
Schoofs, Liliane #
Issue Date: Mar-2009
Publisher: Elsevier
Series Title: Peptides vol:30 issue:3 pages:449-457
Abstract: Neuropeptides are important signaling molecules that function in cell-cell communication as neurotransmitters or hormones to orchestrate a wide variety of physiological conditions and behaviors. These endogenous peptides can be monitored by high throughput peptidomics technologies from virtually any tissue or organism. The neuropeptide complement of the soil nematode Caenorhabditis elegans has been characterized by on-line two-dimensional liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (2D-nanoLC Q-TOF MS/MS). Here, we use an alternative peptidomics approach combining liquid chromatography (LC) separation with matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry to map the peptide content of C. elegans and another Caenorhabditis species, Caenorhabditis briggsae. This study allows a better annotation of neuropeptide-encoding genes from the C. briggsae genome and provides a promising basis for further evolutionary comparisons.
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Microbial and Molecular Systems - miscellaneous
European Centre for Ethics
× corresponding author
# (joint) last author

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