Title: Expression, renaturation and functional analysis of an excitatory insect-specific toxin from scorpion Buthus martensii Karsch
Authors: Li, Chong ×
Liu, Wei
Bosmans, Frank
Zhu, Rong-Huan
Tytgat, Jan
Wang, Da-Cheng #
Issue Date: Oct-2005
Series Title: Protein and peptide letters vol:12 issue:7 pages:635-8
Abstract: The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.
ISSN: 0929-8665
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Toxicology and Pharmacology
× corresponding author
# (joint) last author

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