Author:

Keywords:

Anti-Bacterial Agents, Azithromycin, Chromatography, Liquid, Reproducibility of Results, Sensitivity and Specificity, Spectrophotometry, Ultraviolet, Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Chemistry, Analytical, Biochemistry & Molecular Biology, Chemistry, ANTIBIOTIC AZITHROMYCIN, ERYTHROMYCIN SERIES, HUMAN SERUM, DERIVATIVES, SEPARATION, INVITRO, ASSAY, 0301 Analytical Chemistry, 0399 Other Chemical Sciences, Analytical Chemistry, 3401 Analytical chemistry

Abstract:

An isocratic liquid chromatographic method with UV detection at 215 nm, which is suitable for the analysis of azithromycin (AZT) in bulk samples, is described. AZT is separated from its synthesis intermediates and a degradation product as well as from six unknown impurities on an XTerra RP18 column at 70 degrees C using a mobile phase consisting of acetonitrile-pH 6.5 0.2M K2HPO4-water (35:10:55, v/v/v) at 1.0 mL/min. The XTerra stationary phase contains methyl groups that are incorporated in the bulk structure of the material. This allows for special selectivities. Robustness is evaluated by a full factorial design experiment. The method shows good selectivity, repeatability, linearity, and sensitivity.